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Cyclic Expression of A Nuclear Protein In A Dinoflagellate
Author(s) -
BHAUD YVONNE,
GÉRAUD MARIELINE,
AUSSEIL JÉRǑME,
SOYERGOBILLARD MARIEODILE,
MOREAU HERVE
Publication year - 1999
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1999.tb05123.x
Subject(s) - biology , dinoflagellate , protein expression , expression (computer science) , computational biology , nuclear protein , microbiology and biotechnology , evolutionary biology , genetics , botany , gene , transcription factor , computer science , programming language
. Nuclei of the dinoflagellate Crypthecodinium cohnii strain Whd were isolated and nuclear proteins were extracted in three fractions, corresponding to the increasing affinity of these proteins to genomic DNA. One fraction contained two major bands (48‐ and 46‐kDa) and antibodies specific to this fraction revealed two major bands by Western blot on nuclear extracts, corresponding to the 46‐ and 48‐kDa bands. the 48‐kDa protein was detected in G1 phase but not in M phase cells. an expression cDNA library of C. cohnii was screened with these antibodies, and two different open reading frames were isolated. Dinoflagellate nuclear associated protein (Dinapl), one of these coding sequences, was produced in E. coli and appeared to correspond to the 48‐kDa nuclear protein. No homologue of this sequence was found in the data bases, but two regions were identified, one including two putative zinc finger repeats, and one coding for two potential W/W domains. the second coding sequence showed a low similarity to non‐specific sterol carrier proteins. Immunocytolocalization with specific polyclonal antibodies to recombinant Dinapl showed that the nucleus was immunoreactive only during the G1 phase: the nucleoplasm was immunostained. while chromosome cores and nuclear envelopes were negative.