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The Small Subunit Ribosomal RNA Gene Sequence of Pleistophora anguillarum and The Use of PCR Primers for Diagnostic Detection of the Parasite
Author(s) -
Hung HsuWei,
Lo ChuFang,
Tseng ChiehChih,
Peng ShaoEn,
Chou Chih Ming,
Kou GuangHsiung
Publication year - 1998
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1998.tb05116.x
Subject(s) - biology , ribosomal rna , parasite hosting , gene , protein subunit , genetics , gene sequence , rna , microbiology and biotechnology , 16s ribosomal rna , world wide web , computer science
Using the polymerase chain reaction (PCR) and two primers for conserved regions of the small subunit ribosomal RNA (SSU‐rRNA.) of Microsporidia, a DNA segment about 1,195 base pairs long was amplified from a DNA template prepared from purified spores of the microsporidian species Pleistophora anguillarum. These spores had been isolated from adult eels ( Anguilla japonica ) with “Beko Disease.” A comparison of sequence data from other microsporidian species showed P. anguillarum SSU‐rRNA to be most similar to Vavraia oncoperae. When juvenile eels were artificially infected with P. anguillarum , enzyme‐linked immunosorbent assay could detect a positive infection only 12 days post‐infection. However, when suitable PCR primers were used, a DNA fragment of about 0.8 kb was detected from these juvenile eels after only 3 days post‐infection. No PCR product was obtained with templates prepared from clinically healthy control animals.