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Cultured Rat and Purified Human Pneumocystis carinii Stimulate Intra‐but not Extracellular Free Radical Production in Human Neutrophils
Author(s) -
Jensen Trine,
Aliouat El M,
Lundgren Bettina,
Settnes Osvald P,
Karlsson Anna,
Dahlgren Claes,
DeiCas Eduardo,
Obel Niels
Publication year - 1998
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1998.tb05114.x
Subject(s) - pneumocystis carinii , extracellular , intracellular , biology , superoxide , opsonin , biochemistry , microbiology and biotechnology , immunology , phagocytosis , enzyme , pneumocystis jirovecii , human immunodeficiency virus (hiv)
The production of free radicals in human neutrophils was studied in both Pneumocystis carinii derived from cultures of L2 rat lung epithelial‐like cells and Pneumocystis carinii purified from human lung. Using the cytochrome C technique, which selectively measured extracellular superoxide generation, hardly any free radical production was observed after stimulation with cultured rat‐derived P. carinii. A chemiluminescence technique, which separately measured intra‐ and extracellular free radical production, was subsequently employed to differentiate the free radical generation. It was established that 1) P. carinii stimulated intra‐ but not extracellular free radical production in human neutrophils. 2) opsonized cultured rat‐derived P. carinii stimulated human neutrophils to a strong intra‐cellular response of superoxide production, and 3) opsonized P. carinii. purified from human lung also stimulated human neutrophils to produce intracellular free radicals.