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The Interaction of Acanthamoeba spp. with Activated Macrophages and with Macrophage Cell Lines
Author(s) -
MarcianoCabral Francine,
Toney Denise M.
Publication year - 1998
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1998.tb05099.x
Subject(s) - acanthamoeba , biology , microbiology and biotechnology , macrophage , cytolysis , acanthamoeba keratitis , tumor necrosis factor alpha , macrophage activating factor , in vitro , immunology , cytotoxicity , biochemistry
Acanthamoeba spp. are free‐living amebae associated with amebic keratitis and chronic granulomatous amebic encephalitis. The present studies were undertaken to compare the pathogenicity of three species of Acanthamoeba in B 6 C 3 F 1 mice after intranasal challenge with Acanthamoeba‐induced cytopathogenicity for different macrophage populations. The ability of murine macrophage cell lines and activated murine peritoneal macrophages to lyse Acanthamoeba has been assessed by coincubating macrophages with 3 H‐uridine labeled amebae. Conversely, destruction of macrophages by Acanthamoeba was determined by measuring the release of chro‐mium‐51 from radiolabeled macrophages. Acanthamoeba culbensoni , which is highly pathogenic for mice, destroys macrophage cultures in vitro. Activated primary peritoneal macrophages were more resistant to Acanthamoeba ‐mediated destruction than macrophage cell lines activated in vitro. Activated macrophages were capable of limited destruction of Acanthamoeba polyphaga and Acanthamoeba castellanii. Acanthamoeba ‐specific antibodies increased the amebicidal activity of activated macrophages. Macrophage‐mediated destruction was by contact‐dependent cytolysis and by ingestion of amebae. Conditioned medium obtained from macrophage cultures after treatment with lipopolysaccharide and interferon gamma was neither cytolytic nor cytostatic for Acanthamoeba spp. Purified recombinant cytokines including tumor necrosis factor α. interleukin 1α, and interleukin 1β, alone or in combination, were not cytolytic for Acanthamoeba trophozoites.