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A Short Internal Eliminated Sequence with Central Conserved Sequences Interrupting the LA‐MSC Gene of the 81 Locus in the Hypotrichous Ciliates Oxytricha fallax and O. trifallax
Author(s) -
Seegmiller Adam,
Herrick Glenn
Publication year - 1998
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1998.tb05069.x
Subject(s) - biology , macronucleus , transposable element , locus (genetics) , genetics , gene , coding region , sequence (biology) , conserved sequence , allele , base sequence , mutant
IES‐LA is a short Internal Eliminated Sequence interrupting LA‐MSC, a protein‐coding gene of the 81 locus of Oxytricha fallax and O. trifallax. IES‐LA is precisely excised from the gene during development of the macronucleus. The internal eliminated sequence is bounded by CAAT… AATG, and thereby resembles a TBE1 transposon internal eliminated sequence insertion that is grossly shortened (4.1 kbp to 52‐64 bp), consistent with the hypothesis that short IESs are degenerated ancient transposons. The pattern of sequence conservation between five alleles of IES‐LA shows that it differs from previously characterized classes of ciliate short IESs: while many short IESs have conserved ends and diverged centers, IES‐LA is more conserved in its center and its ends are diverged. This implies a excision mechanism for IES‐LA that is distinct from those for other known Oxytricha IESs.