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Ubiquinone Synthesis by Pneumocystis carinii : Incorporation of Radiolabeled Polyprenyl Chain and Benzoquinine Ring Precursors
Author(s) -
SUL DONGGENUN,
KANESHIRO EDNA S.
Publication year - 1997
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1997.tb05780.x
Subject(s) - shikimic acid , biology , pneumocystis carinii , biochemistry , mevalonic acid , in vitro , metabolism , terpenoid , benzoquinone , protozoa , stereochemistry , microbiology and biotechnology , biosynthesis , chemistry , virology , enzyme , human immunodeficiency virus (hiv) , pneumocystis jirovecii
SUMMARY Radiolabeled mevalonic acid was incroporated in vitro into Pneumocystis carinii ubiquinone homologs, CoQ 9 and CoQ 10 , dermostrating the isoprenoid branch pathway forming the polyprenyl chain of ubiquinone is functional in the organism. Radiolabeled shikimic acid, tyrosine and p ‐hydroxybenzoic acid were also incorporzated into the two ubiquinone homologs, indicating that P. carnii also possesed the biological pathway for de novo synthesis of the CoQ benzoquinone ring.