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Effects of Dichloroisoproterenol on Macromolecular Synthesis and Differentiation in Tetrahymena vorax
Author(s) -
RYALS PHILLIP E.,
YANG XIANGLI
Publication year - 1997
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1997.tb05689.x
Subject(s) - tetrahymena , biology , vacuole , biosynthesis , biochemistry , ciliata , macromolecule , contractile vacuole , tetrahymena pyriformis , microbiology and biotechnology , protozoa , genetics , gene , cytoplasm
. The effect of dichloroisoproterenol on macrostomal cell formation in Tetrahymena vorax was examined and the drug was found to be 50% inhibitory at a concentration of 88 μM. Cellular uptake and incorporation of a variety of radiolabelled precursors was monitored in the presence of dichloroisoproterenol. The results demonstrate a strong, concentration‐dependent inhibitory effect on RNA and protein biosynthesis, with a lesser inhibition observed for lipid biosynthesis. These data indicate that dichloroisoproterenol's reported effects on vacuole formation and processing in Tetrahymena are nonspecific with regard to phagocytic processes, but result from a general suppression of macromolecular synthesis.