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Combined Action of Inhibitors of S‐Adenosylmethionine Decarboxylase with an Antimalarial Drug, Chloroquine, on Plasmodium falciparum
Author(s) -
DAS BHAGWAN,
GUPTA RASHMI,
MADHUBALA RENTALA
Publication year - 1997
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1997.tb05683.x
Subject(s) - chloroquine , plasmodium falciparum , biology , pharmacology , methylglyoxal , in vitro , drug action , drug , biochemistry , enzyme , malaria , immunology
. Methylglyoxal bis (guanylhydrazone), (MGBG) a potent competitive inhibitor of S‐adenosyl‐L‐methionine decarboxylase activity, Berenil, a trypanocidal agent and chloroquine, the commonly used antimalarial resulted in a dose dependent inhibition of Plasmodium falciparum in vitro. The IC 50 values of MGBG, Berenil and chloroquine were 224 μM, 40 μM and 42 nM respectively. Parasites treated with different concentrations of MGBG or Berenil were arrested at the trophozoite stage of the erythrocytic cycle. The combined action of chloroquine (10 nM) with either Berenil (0.1 mM) or MGBG (0.1 mM) on P. falciparum growth showed an additive inhibitory effect. The effect of these inhibitors alone and in combination on polyamine biosynthesis is also reported.