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Gram‐Chromotrope: a New Technique that Enhances Detection of Microsporidial Spores in Clinical Samples
Author(s) -
MOURA HERCULES,
SILVA JORGE L. NUNES DA,
SODRÉ FERNANDO C.,
BRASIL PATRICIA,
WALLMO KRISTY,
WAHLQUIST SUSANNE,
WALLACE SARA,
CROPPO GIAN P.,
VISVESVARA GOVINDA S.
Publication year - 1996
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1996.tb05019.x
Subject(s) - staining , spore , microsporidia , biology , microbiology and biotechnology , gram staining , gram , bacteria , antibiotics , genetics
SUMMARY We have developed a new staining procedure that combines the traditional Gram staining for bacteria and the Weber's chromotrope staining method, the standard technique for the detection of microsporidia spores in clinical Specimens. This “Gram‐chromotrope” staining technique enhances the staining characteristics of microsporidia spores and facilitates the easy detection and differentiation of spores from other microorganisms that are found in clinical specimens, especially stool samples. This new technique is fast, reliable, and simple to perform, and can be easily adapted for use in clinical laboratories.