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Detailed Analyses of Individual Neutral Lipid Classes of Pneumocystis
Author(s) -
KALLAM KATHRYN A.,
KANESHIRO EDNA S.
Publication year - 1996
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1996.tb04986.x
Subject(s) - pneumocystis carinii , biology , phospholipid , fatty acid , biochemistry , gas chromatography , thin layer chromatography , chromatography , fatty alcohol , chemistry , immunology , human immunodeficiency virus (hiv) , membrane , pneumocystis jirovecii
SUMMARY Individual neutral lipid classes of Pneumocystis carinii carinii were isolated and purifed by thin‐layer chromatography. The fatty acids in steryl esters, triglycerides, free fatty acids, diglycerides, and monoglycerides were quantified by gas‐liquid chromatography. The fatty acid compositions of these lipids were similar to those of the neutral lipid classes in whole rat lung controls, unlike comparable studies of phospholipid classes. The free fatty alcohols of rat lung controls and P. carinii were also identified and quantified; only saturated fatty alcohols were detected.