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ITSs Typing of P. carinii Samples from Italy, The Netherlands and Tanzania.
Author(s) -
ATZORI CHIARA,
AGOSTONI FIORENZA,
CARGNEL ANTONIETTA,
PERENBOOM ROOS,
BECKERS PETER
Publication year - 1996
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1996.tb04979.x
Subject(s) - biology , tanzania , typing , ribosomal rna , pneumocystis carinii , pneumonia , incidence (geometry) , molecular epidemiology , virology , internal transcribed spacer , genetics , human immunodeficiency virus (hiv) , gene , medicine , geography , genotype , physics , environmental planning , optics , pneumocystis jirovecii
SUMMARY P. carinii molecular epidemiology appears a new interesting investigational field to understand distribution and incidence of isolates from different geographical locations. Recently a typing system, the Type Specific Oligoblotting (TSO) based on 6 different sequences of the Internal Transcribed Spacers (ITSs) of P. carinii rRNA has been developed [1]. By using P. carinii ITSs nested PCR followed by TSO hybridization we have typed 55 lung derived specimens collected in Italy, The Netherlands and sub‐Saharian Africa from pts with microwpically detected P. carinii pneumonia.