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DNA Polymorphism Revealed by Arbitrary Primers Polymerase Chain Reaction Among Blastocystis Strains Isolated from Humans, a Chicken, and a Reptile
Author(s) -
YOSHIKAWA HISAO,
NAGONO ISAO,
YAP EU HIAN,
SINGH MULKIT,
TAKAHASHI YUZO
Publication year - 1996
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1996.tb04492.x
Subject(s) - blastocystis , biology , polymerase chain reaction , primer (cosmetics) , microbiology and biotechnology , strain (injury) , parasite hosting , protozoa , polymorphism (computer science) , virology , dna , genetics , genotype , gene , feces , chemistry , organic chemistry , anatomy , world wide web , computer science
DNA polymorphisms of different strains of Blastocystis isolated from humans, a chicken, and a reptile were examined by an arbitrary primer PCR method. Two strains of Blastocystis hominis isolated from humans in the USA and Japan yielded nearly identical PCR products. However, one strain of B. hominis (isolated from a human in Singapore) yielded quite different PCR products. Blastocystis sp. isolated from a chicken yielded PCR products similar to those of the former two strains, while Blastocystis lapemi , isolated from a reptile, shared no bands with any of the other isolates. These results indicate the possibility that our isolate from the chicken is a zoonotic strain, and that there is intraspecific variation of Blastocystis hominis .