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The Lobster Parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae): Nuclear 18S rDNA Sequence, Phylogeny and Detection Using Oligonucleotide Primers
Author(s) -
RAGAN MARK A.,
CAWTHORN RICHARD J.,
DESPRES BEATRICE,
MURPHY COLLEEN A.,
SINGH RAMA K.,
LOUGHLIN MICHAEL B.,
BAYER ROBERT C.
Publication year - 1996
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1996.tb03998.x
Subject(s) - biology , oligonucleotide , cytosine , ribosomal rna , phylogenetic tree , 18s ribosomal rna , ribosomal dna , nucleic acid sequence , microbiology and biotechnology , phylogenetics , gene , polymerase chain reaction , mitochondrial dna , ciliate , guanosine , genetics
. We have determined the nucleotide sequence of the nuclear gene encoding small‐subunit ribosomal ribonucleic acid of the ciliate Anophryoides haemophila , a parasite of the American lobster Homarus americanus. The gene is 1763 bp in length, and has a guanosine‐plus‐cytosine content of 43.9%. Inferred phylogenetic frameworks strongly support the monophyly of the scuticociliates, and suggest that order Scuticociliatida should be elevated to at least subclass rank. Oligonucleotide probes based on A. haemophila ssu‐rDNA can discriminate between DNAs of A. haemophila and other investigated hymenostome ciliates, and effectively prime polymerase chain reaction‐based detection of A. haemophila deoxyribonucleic acid against at least a 1600‐fold excess of total deoxyribonucleic acid from H. americanus.