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Molecular Cloning and Characterization of a Leishmania donovani α‐Tubulin Gene
Author(s) -
JOSHI MANJU,
DWYER DENNIS M.,
NAKHASI HIRA L.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb05918.x
Subject(s) - biology , leishmania donovani , cloning (programming) , molecular cloning , gene , leishmania , tubulin , genetics , computational biology , leishmaniasis , gene expression , visceral leishmaniasis , parasite hosting , microtubule , computer science , world wide web , programming language
. We have isolated a cDNA for an α‐tubulin mRNA from L. donovani promastigotes and determined its complete nucleotide sequence. Both nucleotide and deduced amino acid sequence analysis of this cDNA showed significant similarity with a previously reported, partial sequence of an L. enriettii α‐tubulin and the complete sequence of human α‐tubulin. Further, the in vitro translated L. donovania α‐tubulin gene product was specifically immunoprecipitated with a monoclonal antibody against human α‐tubulin. Northern blot analysis revealed that there was little change in the expression of the L. donovani α‐tubulin RNA during parasite differentiation from promastigote to the in vitro grown “amastigote” form. Southern blot analysis revealed a simple genomic organization for the L. donovani α‐tubulin gene with more than one copy of the α‐tubulin gene in the parasite genome. To our knowledge, this is the first complete sequence of an α‐tubulin for Leishmania to be reported in the literature.