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Elucidation of the DNA Synthetic Cycle of Entamoeba Spp. Using Flow Cytometry and Mathematical Modeling
Author(s) -
DVORAK JAMES A.,
KOBAYASHI SEIKI,
ALLING DAVID W.,
HALLAHAN CLAIRE W.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb05915.x
Subject(s) - biology , flow cytometry , dna , computational biology , biological system , genetics
. We developed a method to study the DNA synthetic cycles of Entamoeba histolytica and Entamoeba invadens by flow cytometry (FCM) based on a preparative procedure to reduce both high levels of natural fluorescence and non‐specific adsorption of fluorochromes. We modeled G 1 , S, and G 2 phases as a series of overlapping Gaussian curves. Both E. histolytica and E. invadens displayed G 1 , S, and G 2 proportions that are consistent with eukaryotic cell populations in exponential or stationary growth phase. Exponential phase E. histolytica populations contained a hypodiploid subset with a mass of about 20% less than the diploid value which we estimate by FCM to be 24 × 10 ‐14 g DNA/cell. Exponential phase E. invadens populations contained a hypodiploid subset with a mass of about 6% less than the diploid value which we estimate by FCM to be 30 × 10 ‐14 g DNA/cell.