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Cryopreservation and Storage of Parasitic Protozoa in Liquid Nitrogen
Author(s) -
DIAMOND LOUIS S.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb05911.x
Subject(s) - protozoa , biology , cryopreservation , liquid nitrogen , parasite hosting , zoology , microbiology and biotechnology , genetics , embryo , physics , quantum mechanics , world wide web , computer science
. In 1961 I reported the first successful attempt to cryopreserve and store protozoa in liquid nitrogen. In this presentation, drawing on more than 30 years of personal experience, I discuss the factors which I consider to be most critical to the successful preservation and long term storage of several species of parasitic protozoa in liquid nitrogen. I then present my most successful protocol for cryopreservation of these parasites. Finally. I cite some longevity records for a variety of protozoa stored in liquid nitrogen vapor at the Laboratory of Parasitic Diseases. The oldest record is for a monoxenic culture of Entamoeha histolytica grown in association with Crithidia fasciculata that was frozen on January 6, 1961. Thirty‐two years and 11 months later both parasites were recovered and used to initiate new culture lines.