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Acanthamoeba pearcei N. Sp. (Protozoa: Amoebida) from Sewage Contaminated Sediments
Author(s) -
NERAD THOMAS A.,
SAWYER THOMAS K.,
LEWIS EARL J.,
MCLAUGHLIN SHAWN M.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01619.x
Subject(s) - acanthamoeba , biology , protozoa , naegleria , sewage , amoeba (genus) , microbiology and biotechnology , environmental engineering , engineering
. Seabottom sediments from a discontinued Philadelphia‐Camden 40‐Mile ocean sewage disposal site were cultured for cyst‐forming free‐living amoebae. Barge delivered wastes were discharged at the site from 1973 until 1980 when the site was closed. One station at the southeast margin of the site was sampled at a depth of approximately 50 m, twice in 1978 and once in 1982, 1983 and 1984. Sediment from the 1978 collection yielded Acanthamoeba polyphaga, Vahlkampfia sp., and an unknown amoeba with stellate endocysts similar to those of A. astronyxis. Trophozoites and cysts of the isolate were typical of those described for the genus Acanthamoeba. Biochemical tests employing enzyme electrophoresis and morphological studies on live and stained specimens showed that the isolate was distinct from other well‐described species within the family Acanthamoebidae Sawyer & Griffin, 1975.