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Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite)
Author(s) -
GHAZALI MACHHOUR,
RODIER MARIEHELENE,
MOUDNI BRAHIM,
BABIN PHILIPPE,
FERNANDEZ BEATRICE,
JACQUEMIN JEANLOUIS
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01607.x
Subject(s) - rhoptry , spectrin , toxoplasma gondii , biology , immunogold labelling , polyclonal antibodies , monoclonal antibody , immunofluorescence , parasite hosting , microbiology and biotechnology , antibody , apicomplexa , plasmodium falciparum , biochemistry , immunology , cytoskeleton , cell , malaria , world wide web , computer science
. We demonstrated here the presence of proteins antigenically related to human erythroid spectrin in the parasitic protozoan Toxoplasma gondii . A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal and polyclonal anti‐human erythroid spectrin antibodies on electroblotted nitrocellulose sheets. Indirect immunofluorescence assay clearly showed that these proteins were localized in the anterior pole of the organism. Immunogold staining further revealed specific labeling of conoid, rhoptries, micronemes, and dense granules of the apical complex. The presence of the M, 245–240,000 doublet and the M, 75,000 spectrin‐like proteins in the anterior pole of T. gondii may probably be consistent with a structural stabilizer function in its organciles which are suspected to be involved in the process of host cell invasion.