Premium
Cloning and Analysis of a Cryptosporidium parvum Gene Encoding a Protein with Homology to Cytoplasmic Form Hsp70
Author(s) -
KHRAMTSOV NIKOLAI V.,
TILLEY MICHAEL,
BLUNT DENNIS S.,
MONTELONE BETH A.,
UPTON STEVE J.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01605.x
Subject(s) - biology , microbiology and biotechnology , gene , homology (biology) , cryptosporidium parvum , hspa14 , heat shock protein , western blot , hspa2 , molecular cloning , southern blot , sequence analysis , peptide sequence , recombinant dna , polyclonal antibodies , hspa4 , complementary dna , genetics , antibody
. An intronless gene encoding a protein of 674 amino acid residues with a molecular mass of 73,403 Da showing homology to the cytoplasmic form of the 70 kDa heat shock proteins has been cloned and sequenced from the intestinal pathogen Cryptosporidium parvum . Monospecific polyclonal antibodies obtained to recombinant protein recognized a single band with an approximate molecular mass of 70 kDa on a Western blot of C. parvum proteins, as well as the 70 kDa heat shock protein from bovine brain. Southern blot analysis suggested the gene was single copy in the C. parvum genome. Eleven perfect repeats of the sequence GGMP were found in the predicted protein near the carboxyl terminus.