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A Three Nucleotide Signature Sequence in Small Subunit rRNA Divides Human Giardia in Two Different Genotypes
Author(s) -
KEULEN HARRY,
HOMAN WIEGER L.,
ERLANDSEN STANLEY L.,
JARROLL EDWARD L.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01600.x
Subject(s) - biology , 16s ribosomal rna , 23s ribosomal rna , ribosomal rna , genetics , giardia , nucleic acid sequence , genotype , gene , ribosomal dna , microbiology and biotechnology , phylogenetics , rna , ribosome
. The nucleotide sequence of the 16S rRNA gene, part of the 23S rRNA gene and the spacer DNA region was determined for Giardia duodenalis , obtained from humans in The Netherlands (AMC‐4) and Washington State (CM). These rDNA sequences differ from other G. duodenalis isolates (Portland‐1 and BRIS/83/HEPU/106) both of which have virtually identical rDNA sequences. The most characteristic feature was found close to the 5’end of the 16S rRNA. The Portland‐1 ‐ Bris/83/HEPU/106 type has GCG in position 22–24, while AMC‐4 and CM have AUC in this position. These two sequences, present in an otherwise conserved region of the 16S rRNA, are “signature” sequences, which divide Giardia isolates into two different groups.