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Evidence of a Cadmium‐Thionein and the Glycine Cleavage System in Oxytricha granulifera
Author(s) -
IRATO PAOLA,
PICCINNI ESTER,
JAMES PETER,
AMMERMANN DIETER
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01597.x
Subject(s) - cleavage (geology) , biology , cysteine , glycine , amino acid , biochemistry , cadmium , aromatic amino acids , enzyme , chemistry , organic chemistry , paleontology , fracture (geology)
. This work presents the further purification of a Cd‐linking protein in Oxytricha granulifera by reverse‐phase chromatography. This protein contains 25% cysteine and no aromatic amino acid. it may be considered as a chelatin with some similarity to metallothioneins. During the purification of another Cd‐linking compound, we were able to demonstrate that the H protein precursor of glycine cleavage is present in Oxytricha. This is the first finding of the presence of this system in Protozoa.