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Long‐Range Restriction Mapping of Megabase‐Sized Chromosomes that May Be Homologs in Trypanosoma brucei
Author(s) -
BASTIAN L. SCOT,
LODES MICHAEL J.,
MYLER PETER J.,
STUART KENNETH
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01580.x
Subject(s) - biology , trypanosoma brucei , homologous chromosome , genetics , range (aeronautics) , evolutionary biology , gene , materials science , composite material
.Trypanosoma brucei is a blood‐borne pathogen that changes its variant surface glycoprotein coat, thus evading immune destruction. Restriction digestion, combined with probe hybridization studies, was used to construct long‐range restriction maps of the 1.4 (M4) and 1.5 megabase (M3) chromosomes from the IsTaR1 serodeme of T. b. brucei . Comparison of the two chromosomes suggests that they are a homologous pair. Hybridization with a repetitive sequence probe also identifies several copies on the M4 chromosome and a relative paucity of cross‐hybridizing repetitive sequence on the larger M3 chromosome.