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Phylogenetic Relationships Among Anuran Trypanosomes as Revealed by Riboprinting
Author(s) -
CLARK C. GRAHAM,
MARTIN DONALD S.,
DIAMOND LOUIS S.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01546.x
Subject(s) - biology , phylogenetic tree , intraspecific competition , restriction enzyme , phylogenetics , ribosomal rna , sister group , ribosomal dna , taxon , zoology , restriction site , genetics , evolutionary biology , clade , gene , ecology
Twenty trypanosome isolates from Anura (frogs and toads) assigned to several species were characterized by riboprinting–restriction enzyme digestion of polymerase chain reaction amplified small subunit ribosomal RNA genes. Restriction site polymorphisms allowed distinction of all the recognized species and no intraspecific variation in riboprint patterns was detected. Phylogenetic reconstruction using parsimony and distance estimates based on restriction fragment comigration showed Trypanosoma chattoni to be only distantly related to the other species, white T. ranarum and T. fallisi appear to be sister taxa despite showing non‐overlapping host specificities.