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Comparison of Coding and Spacer Region Sequences of Chromosomal rRNA‐Coding Genes of Two Sequevars of Pneumocystis carinii
Author(s) -
ORTIZRIVERA MARIA,
LIU YONG,
FELDER REGINA,
LEIBOWITZ MICHAEL J.
Publication year - 1995
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1995.tb01538.x
Subject(s) - biology , internal transcribed spacer , intron , pneumocystis carinii , genetics , gene , ribosomal rna , polymerase chain reaction , coding region , spacer dna , ribosomal dna , rna splicing , restriction map , nucleic acid sequence , phylogenetics , rna , virology , human immunodeficiency virus (hiv) , pneumocystis jirovecii
Two distinct sequevars, denoted Pc1 and Pc2, of the opportunistic pathogen Pneumocystis carinii have been previously identified based on the sequence of their 26S rRNA genes, the location of group I self‐splicing introns and pulsed field electrophoretic patterns of chromosomal DNA. This study shows that the sequences of 16S and 5.8S rRNA genes also vary between these sequevars, and that greater variation was seen in the internal transcribed spacer regions. Polymerase chain reaction and restriction analysis can distinguish between these sequevars.