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Identification of Precystic Stages and Encystment Kinetics Analysis of Colpoda inflata by Using an Immunofluorescent Method
Author(s) -
BENITEZ LAURA,
MARTINGONZALEZ ANA,
GUTIERREZ JUAN C.
Publication year - 1994
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1994.tb06094.x
Subject(s) - ciliate , biology , antiserum , polyclonal antibodies , kinetics , cyst , identification (biology) , ecology , immunology , pathology , antibody , medicine , physics , quantum mechanics
. The application of an immunocytochemical method to identify precystic stages and to analyse the encystment kinetics, by using a polyclonal antiserum against isolated cyst walls from the ciliate Colpoda inflata , is reported for the first time. Three different precystic phases were chosen on the basis of morphological changes and degree of cyst wall formation. By using this procedure a better identification of mature resting cysts with regard to precystic cells or young cysts is provided. An average consensus encystment kinetics of C. inflata , by using an accumulated class frequency analysis, is reported.