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F‐actin Distribution of Dictyostelium Myosin I Double Mutants
Author(s) -
PETERSON MICHELLE D.,
TITUS MARGARET A.
Publication year - 1994
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1994.tb01529.x
Subject(s) - dictyostelium discoideum , dictyostelium , biology , microbiology and biotechnology , myosin , actin , cytoskeleton , amoeba (genus) , cell , biochemistry , gene
The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum . Cells lacking different myosin I pairs (myoA ‐ /myoB ‐ , myoB ‐ /myoC ‐ , and myoA ‐ /myoC ‐ ) were examined with respect to their cytoskeletal organization. F‐actin localization by rhodamine‐phalloidin staining of cells indicates that the myoA ‐ /myoB ‐ , myoB ‐ /myoC ‐ , and myoA ‐ /myoC ‐ cells appear to redistribute their F‐actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.