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A Tandem Repeat of Rat‐derived Pneumocystis carinii Genes Encoding the Major Surface Glycoprotein
Author(s) -
SUNKIN SUSAN M.,
STRINGER SAUNDRA L.,
STRINGER JAMES R.
Publication year - 1994
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1994.tb01509.x
Subject(s) - biology , gene , genetics , complementary dna , intron , open reading frame , pneumocystis carinii , coding region , genome , intergenic region , tandem repeat , genomic organization , peptide sequence , virology , human immunodeficiency virus (hiv) , pneumocystis jirovecii
. A fragment from the genome of rat‐derived Pneumocystis carinii was found to contain two MSG genes arranged as a direct repeat. The sequences from one gene (MSG B), the region between the two genes, and part of the second gene (MSG A) were determined. The two MSG genes were not identical in sequence. The open reading frames of MSG A and MSG B encode non‐identical proteins, both of which are similar to that encoded by a previously published cDNA. The MSG B gene sequence showed no evidence of introns. The 5’and 3’untranslated regions of the MSG gene pair were highly conserved, but the regions immediately upstream of the open reading frames of MSG A and B were different from the region upstream of a previously characterized MSG cDNA. Primers designed to extend upstream of the 5’end of MSG and downstream of the 3’end of MSG were used in a polymerase chain reaction with total genomic P. carinii DNA as template. Presumptive intergenic amplification products from this reaction were cloned and sequenced. The sequences of these regions were similar but distinct, indicating that tandem arrangement of MSG genes is a common organizational motif.