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Comparative Study of Microsporidian Spores by Flow Cytometric Analysis
Author(s) -
AMIGÓ JOSEP M.,
GRACIA MARIAPILAR,
COMAS JAUME,
SALVADÓ HUMBERT,
VIVARÈS CHRISTIAN P.
Publication year - 1994
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1994.tb01499.x
Subject(s) - propidium iodide , biology , spore , microsporidia , flow cytometry , staining , zoology , microbiology and biotechnology , genetics , apoptosis , programmed cell death
. Spore suspensions of microsporidian parasites of fish (Microsporidium ovoideum, Glugea stephani, Glugea atherinae and Spraguea lophii ) have been analyzed by flow cytometry. Spore nuclei were dyed either by propidium iodide or bis‐benzimide (Hoechst 33342). By observation of forward light scatter and fluorescence the four species could be distinguished and the mono‐ and diplokaryotic populations of S. lophii identified. Staining of DNA by bis‐benzimide was better and easier than propidium iodide. Forward light scatter and fluorescence values were characteristic of each species and remained unchanged throughout the year, so flow cytometry can be used for distinction of spores of some microsporidian parasites once their flow cytometric parameters are known. However, special care has to be taken in tool calibration and material preparation for analysis because of the high precision of the technique.