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Effects of Host Cell Density on Cell Infection Level in Antheraea eucalypti (Lepidoptera: Saturniidae) Cell Cultures Persistently Infected with Nosema bombycis (Microsporida: Nosematidae)
Author(s) -
YASUNAGA CHISA,
FUNAKOSHI MASAKO,
KAWARABATA TAKESHI
Publication year - 1994
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1994.tb01486.x
Subject(s) - biology , exigua , saturniidae , lepidoptera genitalia , spore , antheraea pernyi , nosema , microbiology and biotechnology , host (biology) , microsporidia , parasite hosting , botany , spodoptera , ecology , recombinant dna , genetics , world wide web , gene , computer science
. Spores of Nosema bombycis Y9101, isolated from the beet armyworm, Spodoptera exigua , were primed with an alkaline solution and inoculated into Antheraea eucalypti cell cultures. Infected cells were subcultured every five days at three cell densities (2.5 × 10 3 , 5.0 × 10 3 , and 1.0 × 10 4 cells/cm 2 ). A difference was observed in the spread of N. bombycis Y9101 infection between low‐density and higher‐density cultures of host cells. The host cell density did not affect the productivity of secondary infective forms of the parasite. The principal factor determining the rate of microsporidian infection in vitro was the number of host cells existing within the reach of extruded short‐coiled polar tubes from spores germinated intracellularly.