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Hydrolase Compartmentalization Limits Rate of Digestion in Acanthamoeba
Author(s) -
HOHMAN THOMAS C.,
BOWERS BLAIR
Publication year - 1993
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1993.tb06112.x
Subject(s) - phagosome , biology , compartmentalization (fire protection) , acid phosphatase , microbiology and biotechnology , vacuole , biochemistry , digestion (alchemy) , cytochemistry , phagocytosis , enzyme , cytoplasm , chemistry , chromatography
. The kinetics of lysosomal enzyme acquisition by newly formed phagosomes was studied by following the rate of digestion of radiolabeled yeast fed to Acanthamoeba . The distribution of hydrolases among phagosomes was assessed by electron microscopic acid phosphatase cytochemistry and by measurement of three glycosidases in isolated early and late phagosomes. The results show that compartmentalization of hydrolases limit the digestion of large phagocytic loads. The hydrolases appear to be sequestered into the early phagosomes and not to be distributed either by small vesicle transport or phagosome‐phagosome fusion to those formed later. We infer from these results that newly internalized surface membrane in phagosomes is not rapidly randomized with internal pools, but is recycled to the surface as a function of the digestive process.