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Distribution of Macromolecular Methylations in Promastigotes of Leishmania donovani and Impact of Sinefungin
Author(s) -
LAWRENCE FRANÇOISE,
ROBERTGERO MALKA
Publication year - 1993
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1993.tb06111.x
Subject(s) - biology , leishmania donovani , in vitro , biochemistry , methylation , leishmania , methyltransferase , in vivo , nucleoside , parasite hosting , leishmaniasis , genetics , dna , visceral leishmaniasis , world wide web , computer science
. Sinefungin, an antifungal and antiparasitic nucleoside antibiotic, is a very potent antileishmanial agent both in vitro and in vivo. This molecule, structurally related to S‐adenosylmethionine, is a good competitive inhibitor of methyltransferases in vitro. The aim of this report is to analyze the impact of sinefungin on methylation pattern and the subcellular localisation of methyl groups and various methylases in promastigotes of Leishmania donovani . We have shown the presence of various methylated macromolecules in different subcellular fractions, with somewhat higher concentration in membrane fraction. In vitro, sinefungin inhibits the three main protein methylases, but in cells cultured in its presence the protein carboxylmethylations are specifically inhibited.