Premium
Inhibition of Dna Synthesis In the Macronuclear Replication Band of Euplotes Eurystomus
Author(s) -
OLINS DONALD E.,
OLINS ADA L.
Publication year - 1993
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1993.tb04939.x
Subject(s) - aphidicolin , biology , dna replication , dna , microbiology and biotechnology , dna synthesis , calmodulin , phosphodiesterase , dna polymerase , biochemistry , enzyme
. The replication band is a large, migrating, macronuclear domain that is the site of DNA synthesis in hypotrichous ciliated protozoa. A number of agents that produce inactivation of this structure and its replicational activity are described here. These agents include heat shock, aphidicolin, cell crowding, various cAMP phosphodiesterase inhibitors and a calmodulin inhibitor. With the exception of aphidicolin, which has a direct inhibitory effect upon DNA polymerases, the mechanisms of inactivation are presently unknown. the inactivating properties of cAMP phosphodiesterase inhibitors suggest that intracellular cAMP levels may influence replication band structure and function.