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Effect of Low Temperature On the In Vitro Growth of Plasmodium falciparum
Author(s) -
ROJAS MARÍA O.,
WASSERMAN MOISÉS
Publication year - 1993
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1993.tb04895.x
Subject(s) - biology , plasmodium falciparum , incubation , parasite hosting , protozoa , in vitro , incubation period , methionine , plasmodium (life cycle) , apicomplexa , microbiology and biotechnology , immunology , biochemistry , malaria , amino acid , world wide web , computer science
. The effect of low incubation temperature on synchronized cultures of Plasmodium falciparum was studied. Young trophozoites that were maintained at 28°C matured slowly and invaded poorly. Growth seemed to arrest when parasites reached a maturation equivalent to 30 h, although they reestablished their growth normally when returned to 37°C. On the other hand, 36‐h synchronized parasites that were transferred to 28°C completed their cell cycle with a 12‐16 h delay, but without changes in the parasite as seen by light microscopy and without a diminution in the efficiency of the invasion or in the incorporation of 35 S‐methionine. These results might be useful for obtaining parasites at defined stages of development at the desired time.