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Effects of Cadmium in Stylonychia lemnae , Stylonychia notophora and Oxytricha granulifera : Isolation of a Cadmium‐Binding Protein
Author(s) -
PICCINNI ESTER,
IRATO PAOLA,
CAVALLINI LETIZIA,
AMMERMANN DIETER
Publication year - 1992
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1992.tb04855.x
Subject(s) - cadmium , metallothionein , cysteine , chemistry , biochemistry , amino acid , metal , chelation , absorption (acoustics) , metalloprotein , enzyme , organic chemistry , physics , acoustics
The effects of cadmium on three ciliates are reported here. Cultures of Stylonychia lemnae, Stylonychia notophora and Oxytricha granulifera were treated with different doses of Cd according to tolerance. The two species of Stylonychia are very sensitive to the metal, white O. granulifera tolerates higher doses. Adding 50 μM of Cd to the medium did not damage cells. The accumulated metal is almost totally present in the particulate fraction after day 3. Two Cd‐Zn linking fractions were separated from the soluble fraction of culture treated on day 1. The first protein linking 17 μg Cd/mg showed an ultraviolet absorption spectrum similar to that of Cd‐thioneins. Preliminary amino acid analyses indicated that it contained 13% cysteine. The second protein, linking 60 μg Cd/mg, was a glycoprotein. Its ultraviolet absorption spectrum and amino acid analysis showed that this binding protein was far from being a metallothionein: its cysteine content was very low and aromatic and cyclic residues were present. This Cd‐linking compound seems to be unique, since it was very different both from metallothioneins and chelatins isolated by other protozoa. The protective role of these chelating proteins is discussed.