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Development of Plasmodium berghei Ookinetes to Young Oocysts In Vitro
Author(s) -
ARAKAWA RYO,
KAMIMURA KIYOSHI,
KAWAMOTO FUMIHIKO
Publication year - 1992
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1992.tb01325.x
Subject(s) - plasmodium berghei , biology , plasmodium (life cycle) , in vitro , microbiology and biotechnology , clone (java method) , vacuole , cytoplasm , aedes albopictus , gametocyte , cell division , protozoa , virology , plasmodium gallinaceum , cell , immunology , aedes aegypti , parasite hosting , genetics , botany , plasmodium falciparum , malaria , gene , world wide web , computer science , larva
. The mosquito stage of Plasmodium berghei was cultivated in vitro, with special attention to ookinete transformation into early oocyst. The ookinetes were obtained by in vitro culture of gametocytes taken from infected mice, purified by density gradient of metrizoic acid or a lymphocyte separation medium, and incubated either in acellular culture or in co‐cultivations with mosquito cells. In acellular culture, the ookinetes were found to aggregate with each other and transformed from banana to round shapes. Their inner pellicular membranes and subpellicular microtubules partially disappeared, indicating that development to early oocyst had occurred. Co‐cultivation with Aedes albopictus cells (C6/36 clone) revealed that ookinetes transformed into early oocyst in the medium, or invaded the cells and then transformed to early oocysts within the cell cytoplasm as well. However, all of these transformed cells failed to develop further, i.e. neither deposition of the oocyst capsule nor nuclear division was observed. Many ookinetes which failed to penetrate the Aedes cells were phagocytized within three days of culture. A significant difference between invaded and transformed oocysts and phagocytized ookinetes was seen in that the former lacked vacuole membrane. Co‐cultivation with Toxorhynchites amboinensis cells (TRA‐284‐SFG clone) permitted transformation of ookinetes into early oocysts in the medium as in the acellular culture, but no ookinete invasion nor phagocytosis by the cell was observed.