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A Nuclear‐Envelope‐Specific Protein In Amoeba Proteus Detected With A Monoclonal Antibody
Author(s) -
CHOI EUI Y.,
JEON KWANG W.
Publication year - 1991
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1991.tb04808.x
Subject(s) - amoeba proteus , nuclear lamina , cytoplasm , amoeba (genus) , envelope (radar) , monoclonal antibody , nuclear protein , biology , mitosis , immunofluorescence , electron microscope , chemistry , microbiology and biotechnology , antibody , biochemistry , physics , immunology , telecommunications , radar , gene , transcription factor , computer science , optics
A protein with two subtypes of 205 and 180 kDa was localized on the nuclear envelope of amoebae as detected by indirect immunofluorescence staining and immuno‐electron microscopy using a monoclonal antibody as a probe. Electron microscopic observation showed that the protein was located on the honeycomb lamina of the nuclear envelope. During mitosis, the protein dispersed throughout the cytoplasm but reappeared on the nuclear envelope after the reformation of the envelopes of daughter nuclei. the findings suggested that the protein is a component of the nuclear lamina of amoebae.