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Tetrahymena Thermophila : Growth In Synthetic Nutrient Medium In the Presence and Absence of Glucose
Author(s) -
SZABLEWSKI L.,
ANDREASEN P. HOVE,
TIEDTKE A.,
FLORINCHRISTENSEN J.,
FLORINCHRISTENSEN M.,
RASMUSSEN L.
Publication year - 1991
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1991.tb04802.x
Subject(s) - tetrahymena , nutrient , strain (injury) , composition (language) , doubling time , chemically defined medium , growth medium , biology , chemistry , liquid medium , multiplication (music) , biochemistry , food science , cell , bacteria , chromatography , genetics , in vitro , ecology , mathematics , anatomy , linguistics , philosophy , combinatorics
This report contains the newest directions for preparation of the synthetic nutrient medium for some Tetrahymena species that do not require lipids. In the standard medium T. thermophila. strains SB 210 and 281, multiply at 37°C with doubling times of around 2 h and at 26°C around 5 h. We have established multiplication rates as functions of variations in the composition of the medium. In media in which all components are present at one‐third of the normal concentrations and only the essential amino acids are included, growth and multiplication become sharply dependent on glucose in strain SB 281. Such media may be used for selection and enrichment of certain specified cell lines.