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A Serum‐free, Partly Defined Medium, PDM‐805, for Axenic Cultivation of Entamoeba histolytica Schaudinn, 1903 and other Entamoeba
Author(s) -
DIAMOND LOUIS S.,
CUNNICK CAROL C.
Publication year - 1991
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1991.tb04430.x
Subject(s) - entamoeba histolytica , bovine serum albumin , biochemistry , axenic , thioctic acid , ascorbic acid , chemistry , serum albumin , casein , thiamine , biology , microbiology and biotechnology , food science , lipoic acid , bacteria , genetics , antioxidant
We describe the first serum‐free, partly defined medium (PDM‐805) for cultivating the human enteric pathogen, Entamoeba histolytica , and the reptilian amebae E. barreti, E. invadens , and E. terrapinae. PDM‐805 was developed by the stepwise replacement of yeast extract, bovine serum, and a casein peptone digest in TYI‐S‐33, a medium widely used for the axenic cultivation of these parasites. The defined components include amino acids, carbohydrates, B vitamins, ascorbic acid, tocopherol, thioctic acid, nucleic acid precursors, trace metals, and phosphate buffers. The undefined components include a highly purified bovine serum albumin, a lipoprotein‐cholesterol solution from bovine serum, and a dialyzable, autoclavable, water‐soluble growth factor(s) having a molecular weight of less than 3,500 prepared from casein peptone. To date, studies on the growth requirements of E. histolytica , strain 200:NIH, show the following are essential for sustained multiplication of this ameba: iron, glucose, biotin, folic acid, niacinamide, pantothenate, pyridoxal, riboflavin, thiamine, cysteine, an ammonium moiety (in addition to that present in cysteine), bovine serum albumin, lipoprotein‐cholesterol, and casein peptone dialysate.