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Three‐Dimensional Reconstruction of Rabbit‐Derived Pneumocystis carinii from Serial‐Thin Sections I: Trophozoite
Author(s) -
PALLUAULT FRANCOIS,
PIETRZYK BRUNO,
DEICAS EDUARDO,
SLOMIANNY CHRISTIAN,
SOULEZ BENOIT,
CAMUS DANIEL
Publication year - 1991
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1991.tb01377.x
Subject(s) - pneumocystis carinii , organelle , endomembrane system , biology , ultrastructure , microbiology and biotechnology , anatomy , human immunodeficiency virus (hiv) , virology , golgi apparatus , endoplasmic reticulum , pneumocystis jirovecii
The highly complex ultrastructural morphology of the endomembrane system in Pneumocystis carinii led us to perform three‐dimensional reconstruction from serial‐thin sections using the CATIA (Conception Assistée Tridimensionnelle Inter Active) Dassault system program. The three‐dimensional reconstruction of a small trophozoite made it possible to better understand the morphological relationship among organelles and to suggest cytophysiological hypotheses. By reconstructing other parasite stages, we gathered information about the evolution of organelles during the life cycle and about their physiology.