Premium
Further Studies on Substrates Inducing Metacyclogenesis in Trypanosoma cruzi
Author(s) -
KRASSNER STUART M.,
GRANGER BARBARA,
PHERMSANGNGNAM PAT,
LE TUAN,
LINDEN VIC
Publication year - 1990
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1990.tb05882.x
Subject(s) - trypanosoma cruzi , proline , transformation (genetics) , glutamine , malonic acid , phosphoenolpyruvate carboxykinase , biology , fructose , biochemistry , asparagine , microbiology and biotechnology , amino acid , enzyme , parasite hosting , world wide web , computer science , gene
Epimastigotes of Trypanosoma cruzi , Peru strain, incubated in Contreras' artificial triatomine urine transformed into metacyclic trypomastigotes when 10 mM L‐glutamine, L‐asparagine or D‐fructose was added to the medium. Metacyclogenesis with these substrates was comparable to the percent metacyclic morphotype formation induced by L‐proline and significantly greater than that stimulated by 10 mM D‐glucose. Sodium acetate (10 mM) increased transformation induced by L‐proline, and L‐hydroxyproline (10 mM) increased transformation induced by D‐fructose. Phosphoenolpyruvate (10 mM) inhibited L‐proline‐induced metacyclic trypomastigote stage formation. Three antimetabolites, azetidine 2‐carboxylate (5 mM), malonic acid (1 mM), and desthiobiotin (5 mM), completely inhibited D‐fructose‐induced but not L‐proline‐induced transformation. The Costa Rica, Y, and CL strains of T. cruzi showed different patterns of percent metacyclogenesis with substrates that induce transformation in the Peru strain.