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Characterization of Mendelian and Non‐Mendelian Mutant Strains by Micronuclear Transplantation in Paramecium tetraurelia
Author(s) -
KOBAYASHI SUMIKO,
KOIZUMI SADAAKI
Publication year - 1990
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1990.tb01253.x
Subject(s) - micronucleus , biology , macronucleus , micronucleus test , mutant , genetics , cytoplasm , mendelian inheritance , microbiology and biotechnology , gene , transplantation , chemistry , medicine , surgery , organic chemistry , toxicity
. Mutant strain d48 and d12 cannot express serotype A. In d48, the A i‐antigen gene is present in the micronucleus, but not in the macronucleus. It has recently been shown that d12 contains the A gene in its micronucleus, but its macronucleus lacks the gene. Micronuclear transplantations into enucleated cells were performed to analyze those mutants. Reciprocal transplantation between wild type and d48 confirmed that d48 contains the A gene in the micronucleus and its cytoplasm is defective. Wild type 51 enucleated cells into which were transplanted d12 micronuclei could not express A. Amiccronucleate d12 cells into which were transplanted normal micronuclei from 51 or d48 showed no expression of A. These results show that even if the micronucleus of d12 contains the A gene, it must be abnormal, and its cytoplasm is also defective the same as d48. Genetic analysis showed that heterozygote of d12 and wild type 51 or d48 caused a cure of the cytoplasmic defect of d48 and d12 during the development of macronuclei.