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Effect of Gossypol on Trypomastigotes and Amastigotes of Trypanosoma cruzi
Author(s) -
ROVAI LEONOR E.,
AOKI AGUSTIN,
BURGOS NELIA M. GEREZ DE,
BLANCO ANTONCO
Publication year - 1990
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1990.tb01148.x
Subject(s) - gossypol , trypanosoma cruzi , amastigote , incubation , vero cell , intracellular , axenic , biology , motility , bovine serum albumin , microbiology and biotechnology , kinetoplastida , in vitro , biochemistry , leishmania , parasite hosting , bacteria , genetics , world wide web , computer science
Bloodstream Trypanosoma cruzi trypomastigotes isolated from infected mice undergo reduction of motility and structural damages after 5 to 45 min exposure to gossypol at concentrations ranging from 5 to 50 μM. When 1% serum albumin is added to the incubation medium, no alterations of parasites are observed, even with 100 μM gossypol. Intracellular T. cruzi amastigotes in infected Vero cell cultures exposed to 5 μM gossypol for 2 h do not show changes. Incubation with 5 μM gossypol for 48 h produces complete disruption of host cells; however, the amastigotes they contain show only mineor alterations. The observations indicate that, in protein‐rich media, gossypol is complexed into associations which have no activity on the different forms of the T. cruzi biological cycle.