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Specific and Common Epitopes in Mating Pheromones of Euplotes raikovi Revealed by Monoclonal Antibodies
Author(s) -
FIORI PIER LUIGI,
MICELI CRISTINA,
RAFFIONI SIMONA,
VALLESI ADRIANA
Publication year - 1990
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1990.tb01125.x
Subject(s) - sex pheromone , monoclonal antibody , mating , biology , epitope , mating type , pheromone , antibody , immunoperoxidase , antigen , immunology , genetics , gene
Polypeptide mating pheromones E r ‐1 and E r ‐2, purified from the supernatant of Euplotes raikovi cultures of mating type I and mating type II, respectively, were used to immunize mice and obtain monoclonal antibodies. Five hybridoma clones producing antibodies specific to the mating pheromones were selected. They were analyzed for immunospecificity by immunoperoxidase assay, immunoblotting, and for their efficacy in inhibition of mating pheromone activity. Monoclonal antibodies from two hybridoma clones recognized only the mating pheromone used as antigen; those from the other three clones reacted, to comparable extents, with both mating pheromones. On the basis of these results it was assumed that two immunogenic sites exist in E r ‐1 and E r ‐2, one specific and the other common to both mating pheromones.