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S‐Antigen Localization in the Erythrocytic Stages of Plasmodium falciparum
Author(s) -
CULVENOR JANETTA G.,
CREWTHER PAULINE E.
Publication year - 1990
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1990.tb01117.x
Subject(s) - immunoelectron microscopy , antigen , cytoplasm , plasmodium falciparum , biology , antibody , vacuole , vesicle , virology , microbiology and biotechnology , malaria , biochemistry , membrane , immunology
Localization of the S‐antigen of Plasmodium falciparum isolate FCQ27/PNG, from Papua New Guinea, was studied by post‐embedding immunoelectron microscopy using affinity‐purified rabbit antibodies raised against the repeat region of the antigen. Labelling was found in the parasitophorous vacuole (PV) space of early to late schizonts and in PV‐related vesicles within the erythrocyte cytoplasm of schizont‐infected cells. Other subcellular structures within the erythrocyte cytoplasm were not labelled. After breakdown of the PV membrane, label was observed around the merozoites, consistent with mixing of the PV contents and erythrocyte cytoplasm. The antigen was not found in uninfected cells, ring stages, trophozoites or associated with free merozoites. Antibodies to FCQ27/PNG S‐antigen did not react with other isolates tested, whereas rabbit antibodies to the Palo Alto/Wellcome S‐antigen repeat region reacted with isolates FCR3 and ItG2F6 but not with FCQ27/PNG.