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Monoclonal Antibodies Specific for Members of the Genus Endotrypanum
Author(s) -
LOPES ANGELA HAMPSHIRE C. S.,
McMAHONPRATT DIANE
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb05526.x
Subject(s) - monoclonal antibody , crithidia , biology , antigen , antibody , microbiology and biotechnology , immunofluorescence , virology , western blot , dot blot , protozoa , biochemistry , immunology , dna , gene
Twenty‐six monoclonal antibodies were produced against membrane‐enriched preparations of Endotrypanum schaudinni or Endotrypanum sp. promastigotes. Fifteen of these monoclonal antibodies (E1‐E15) reacted only with the standard strain of E. schaudinni , M6159. Monoclonal antibodies E16‐E26 were considered Endotrypanum specific; no cross reactivity was detected with any other genus of the family Trypanosomatidae (Leishmania, Trypanosoma, Leptomonas. Herpetomonas or Crithidia) by dot‐blot radioimmune assay. By indirect immunofluorescence assay, the antigens recognized by Endotrypanum specific monoclonal antibodies appear to be associated with the surface of the parasite. Based on Western blot analysis, 4 antigenic molecules ranging in molecular weight from 24 kD to 160 kD were identified by monoclonal antibodies specific for the strain of E. schaudinni , M6159. Monoclonal antibodies specific for the genus Endotrypanum identified an antigen of molecular weight 48 kD as well as a diffuse component migrating with an apparent molecular weight of 64–200 kD.