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Pneumocystis Antigen Appearance during Immunosuppression
Author(s) -
TVEY M.H.,
DOWNS T.A.,
HART M.C.,
GRAVES D.C.
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb02686.x
Subject(s) - immunosuppression , antigen , monoclonal antibody , immunology , pneumocystis carinii , antibody , biology , monoclonal , virology , microbiology and biotechnology , human immunodeficiency virus (hiv) , pneumocystis jirovecii
The sequential appearance of Pneumocystis carinii (Pc) antigens during the progression of immunosuppression in rats was studied using the immunobloiting technique and specific immunologic probes. Putative Pc soluble antigens, with molecular weights of approximately 70 and 90 kd. were delected as early as 2 wk after initiation of immunosuppression in rats using a pool of monoclonal antibodies produced to I c isolated from lungs using enzymatic digestion. Monoclonal antibodies produced to Pc isolated by massaging the lung tissue using a Stomacher apparatus and infection‐derived sera did not detect soluble antigens until at least the 6th wk of immunosuppression. Analysis of Pc pellets obtained from Stomacher‐ and lavage‐processcd lungs revealed that the lower molecular weight antigens (≤ 40, 45 and possibly 55–60 kd) were recognized early during the immunosuppression process.