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Antigenic Reactivity of a Monoclonal Antibody Against Amoeba proteus Actin
Author(s) -
AHN TAE I.,
JEON KWANG W.
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb01096.x
Subject(s) - amoeba proteus , monoclonal antibody , amoeba (genus) , acanthamoeba , protozoa , antibody , antigen , biology , microbiology and biotechnology , actin , cross reactivity , isoelectric focusing , chemistry , cross reactions , biochemistry , immunology , enzyme
The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross‐reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii , but it did not react with Naegleria gruberi actin. The amoeba actin was resolved into 3 bands with isoeletric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2‐dimensional immunoblots.