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Characterization of a Cytochalasin D‐Resistant Mutant of Entamoeba histolytica
Author(s) -
GARZA MIREYA DE LA,
GALLEGOS BRIGIDO,
MEZA ISAURA
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb01095.x
Subject(s) - mutant , entamoeba histolytica , cytochalasin , wild type , biology , cytochalasin b , actin , microbiology and biotechnology , motility , microfilament , phalloidin , cytochalasin d , cytoskeleton , biochemistry , cell , gene
Characterization of a cytochalasin D‐resistant mutant of the human parasite Entamoeba histolytica capable of growing at 10 μM cytochalasin is described. The mutant cells also show resistance to 5 mM colchicine and 100 μM cytochalasin B, drugs proved deleterious for wild type trophozoites. The mutants show increased osmotic fragility and electric mobility but reduced phagocytic activity, and agglutination by Concanavalin A. On the other hand pinocytic activity remains unaltered when compared with the wild type cells. Polymerized actin, seen by staining with phalloidin, often appears polarized to one end of the trophozoites and forms few of the endocytic invaginations found in wild type amebas. An altered distribution of part of the actin could explain the differences in surface properties and motility observed in the mutant amebas.