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Culture and Generic Identification of Trypanosomatids of Phytophagous Hemiptera in Brazil
Author(s) -
SBRAVATE CLIZETE,
CAMPANER MARTA,
CAMARGO LUIS E. A.,
CONCHON IVETE,
TEIXEIRA MARTA M. G.,
CAMARGO E. PLESSMANN
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb01093.x
Subject(s) - biology , crithidia , hemiptera , lygaeidae , coreidae , pentatomidae , trypanosoma , microbiology and biotechnology , zoology , botany , protozoa , virology
Among the 372 phytophagous Hemiptera examined, 133 insects of 28 species (Coreidae 18, Pentatomidae 7, Pyrrhocoridae 2, Lygaeidae 1) were infected with trypanosomatids. Gut infections only were found in 68.4%, gut and salivary gland infections in 29.3% and salivary infections alone in 2.3%. Fifty‐one cultures were isolated from 38 insects. Cultures were characterized by assay of certain ornithinc‐arginine metabolism enzymes and by indirect immunofluorescence against monoclonal antibodies specific for Phytomonas spp. Ten cultures were identified as either Crithidia or Leptomonas. Twenty‐one promastigote cultures had an enzyme pattern hitherto recorded only for Leishmania and 16 cultures were identified as Phytomonas.