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Plasmodium falciparum Gametocyte Formation In Vitro: Its Stimulation by Phorbol Diesters and by 8‐Bromo Cyclic Adenosine Monophosphateau WILLIAM TRAGER
Author(s) -
GILL GOKAL S.
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb01079.x
Subject(s) - gametocyte , forskolin , plasmodium falciparum , phosphodiesterase , phorbol , in vitro , stimulation , clone (java method) , adenosine , cyclic adenosine monophosphate , chemistry , biology , protein kinase c , microbiology and biotechnology , biochemistry , enzyme , endocrinology , immunology , receptor , malaria , dna
. Phorbol 12‐myristate 13‐acetate increased the number of gametocytes by 50 to 100% in well or petri dish cultures of the HB‐3 clone of Plasmodium falciparum . Phorbol dibutyrate had a similar effect. The optimal concentration for each of these agents was 20 ng/ml or approximately 30 nM. No effect of forskolin was found, other than a general inhibition of growth at concentrations over 10 μM. An inhibitor of phosphodiesterase, 8‐bromo cyclic adenosine monophosphate (at concentrations of 0.1 and 1.0 μM) also significantly increased the number of gametocytes formed by this clone.